These following techniques are all used for the expression of different constructs and proteins involved in the cerebellum patophysiology:
- Western blot;
- Immunoistochemistry and immunofluorescence;
- 3D reconstruction and electroporation
In some experiments we need to use animal models which express constructs coding for the opsins (proteins that can absorbe some components of the light; these components can be stimulate by the system 2PM-SLM).