To understand the natural discharge pattern of neurons, plasticity and resonance, our in-vivo electrophysiological recordings are mainly made in CrusIIa of Cerebellum.
In-vivo stimulation is performed by a Theta Sensory Stimulation (TSS), using an air-puff on the rat whiskerpad.
In some cases also an electrical stimulation can be perfomed into the cerebellum
In-vivo techniques used are the following:
- field recordings (single electtrode);
- patch-clamp recordings (whole-cell and cell-attached configurations);
- MEA recordings (see below)
Example of Multielectrode System (MEA): “16 Electrode Eckhorn Matrix System”
Schematic view of the MEA position on Crus IIA of the Cerebellum and in red the electrode of stymulus
Roggeri L., Rivieccio B., Rossi P., D’Angelo E. (2008). Tactile stimulation evokes long-term synaptic plasticity in the granular layer of cerebellum. The Journal of Neuroscience, 28, pp. 6354-6359.
Diwakar S, Lombardo P, Solinas S, Naldi G, D’Angelo E. (2011). Local field potential modeling predicts dense activation in cerebellar granule cells clusters under LTP and LTD control. PLoS One. 2011;6(7).
D. Gandolfi, P. Lombardo, J. Mapelli, S. Solinas and E. D’Angelo. Theta-frequency resonance at the cerebellum input stage improves spike timing on the millisecond time-scale. Frontiers in Neural Circuits. Vol. 7, article 64. (2013).
- F. Prestori, C. Bonardi, L. Mapelli, P. Lombardo, R. Goselink, M. Egle De Stefano, D. Gandolfi, J. Mapelli, D. Bertrand, M. Schonewille, C. De Zeeuw, E. D’Angelo. Gating of Long-Term Potentiation by Nicotinic Acetylcholine Receptors at the Cerebellum Input Stage. Plos One. May 2013 – Volume 8 – Issue 5 – e64828.